Activity of mitochondrial enzymes under condition of lipid metabolism breach in hepatocytes
L. Kalachnyuk, D. Melínychuk*, G. Kalachnyuk
Biotechnology Research Institute of Animal Productivity
S.Z. Gzhytskyi Líviv National Academy of Veterinary Medicine
Pekarska st. 50, Líviv 79010, Ukraine
*National Agrarian University
Heroyiv Oborony st. 15, Kyiv 03041, Ukraine
It has been shown in monolayer cultures of hepatocytes from healthy calves and from ones with diarrhea that the rise of [1-14C]oleate incorporation rate into acid soluble products, intracellular triacylglicerols and total pool of intracellular lipids depends directly on the [1-14C]oleate enlargement in cultures. The substantial decrease of utilization of [1-14C]oleate by all pointed out molecular forms of lipid metabolism is registered under its supplement with the adding other non-radioactive sources of essential fatty acids and demonstrates the competitive use of lipid components by liver cells. In liver homogenates, it has been found that, under condition of diarrhea, in calf hepatocyte mitochondria, the activity of citrate synthase (which catalyzes initial reaction of the tricarboxylic acid cycle or the TCA cycle) and of cytochrome-c oxidase (that helps to be realized last step of biological oxidation-reduction of molecular oxygen by electrons) is significantly diminished. The found changes are connected with structural and functional breaches, especially with the disturbances of β-oxidation of fatty acids, of the TCA cycle intermediates transformations, of proton transport through inner mitochondrial membrane and of other biochemical processes in hepatocytes.
Keywords: citrate synthase, cytochrome C oxidase, fatty acids, hepatocytes, calves, diarrhea.