Systems of Ca2+ transporting in salivary glands of Drosophila melanogaster larvae

T. Chorna, V. Maníko, M. Klevets

Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine

Ca2+-transport systems were identified in secretory cells in salivary glands of Drosophila melanogaster larvae based on membrane-bound Ca2+ content analysis. It has been shown that ATP (0,1 and 1 mmol/l) caused significant changes of membrane-bound Ca2+ content in glands tissue. Moreover, ATP-induced elevation of Ca2+ content depends on the presence of these ions in extracellular medium. This is an evidence of functional activity of P2X- and P2Y-receptors on the plasma membrane in secretory cells of larval salivary glands. Ryanodine (500 nmol/l) evoked a significant increase of membrane-bound Ca2+ content in salivary glands, which suggests about the existence of ryanodine-sensitive Ca2+-channels in the endoplasmic reticulum membrane. We observed a significant elevation of Ca2+ content in glands tissue under the influence of thapsigargin (1 μmol/l), thereby indicating the functioning of Ca2+-pump in intracellular store membranes.

Keywords: calcium, P2-receptors, ryanodine-sensitive Ca2+-channels, Ca2+-pump, salivary glands, Drosophila melanogaster

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