Cloning and investigation of snorA gene, a putative positive regulator of nogalamycin biosynthesis in Streptomyces nogalater

D. Klymyshin*,**, T. Gren*, V. Fedorenko*

*Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
**Institute of Animal Biology UAAS
38, Stusa St., Lviv 79034, Ukraine
dedima@ rambler.ru

Using polymerase chain reaction the 2,4 kb fragment, containing snorA gene was cloned. The snorA gene was subcloned into pKC1218E vector. Obtained construction was transferred into Streptomyces nogalater IMET43360 cells using bacterial conjugation. It was found that expression of snorA gene in pKC1218E vector in S. nogalater caused increaså in nogalamycin production.


Keywords: Streptomyces nogalater, nogalamycin, a regulation of antibiotic biosynthesis, conjugation


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