Cytochemical analysis of nickel content in cells of moss gametophyte Funaria hygrometrica Hedw.
U. Oksenjuk, O. Lobachevska
Institute of Ecology of the Carpathians of NAS of Ukraine
11, Stefanyk St., Lviv 79000, Ukraine
The influence of nickel ions on the regenerative ability of leaves of moss Funaria hygrometrica Hedw. from various occurrences was investigated. It was established, that the increase of the metal concentration in the nutrient solution led to the inhibition of regenerants development and to the decreasing of the speed of their growth. On the basis of the results of Ni2+ cytochemical determination we showed peculiarities of absorption and localization of nickel in cells after incubation of the moss leaves or their regeneration on agar nutrient medium with in 30, 100, 1000 ÁM NiSO4∙7H2O.
Keywords: nickel, dimethyl glioxim, leaf, ˝ˇtoplasm, vacuole, moss Funaria hygrometrica