Spectral indices of metallothioneins of crucian carp Carrassius auratus as potential biomarker of environmental pollution

H. Falfushynska1, L. Gnatyshyna1, O. Turta1, O. Osadchuk1, M. Kasyanchuk2, O. Stoliar1

1V. Hnatiuk Ternopil National Pedagogical University
Research Laboratory of Comparative Biochemistry and Molecular Biology
2, M. Kryvonis St., Ternopil 46027, Ukraine
2Ternopil National Economical University
11, Lv halynka.f@gmail.com

The spectral properties and metal content in metallothioneins (MTs) isoforms from the liver and gills of crucian carp Carassius auratus resided in clean (Z) and polluted (B) ponds after exposure of copper and manganese ions, as well as organochlorine and dithiocarbamate pesticides (each at two concentrations) during 14 days were compared. Differences between these characteristics of animals from two populations and their changes under exposure to disturbance factors, especially significant in group B due to decreased of metal-binding ability of MTs, especially in the gills were shown. By means of Canonical analysis the general features of UV spectra in fish under effect of natural complex pollution and the model action of pesticides were confirmed.


Keywords: metallothioneins, crucian carp, spectral properties, heavy metals, pesticides


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