Molecular mechanisms in lectin induced signal transduction in human leukocytes under type 1 diabetes mellitus
Ì. Zdioruk, ². Brodyak, N. Sybirna
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
The content of PI-3’-kinase enzyme p85alpha regulatory subunit in leukocytes from healthy donors and patients with type 1 diabetes mellitus was investigated. It was shown that binding of lectins to carbohydrate determinants of membrane glycoconjugates of leukocytes mediated signal transduction through P²-3’-kinase. It was shown that under diabetes cell reaction, which is registered as a translocation of the regulatory subunit of the enzyme PI-3’-kinase after the lectin induced effects on neutrophils and mononuclear cells, was different in intensity and the formation in time for the WGA, SNA, MAA, which may be associated with the quantitative and structural changes in plasma glycoprotein receptors that contain α(2→3)- and α(2→6)-attached terminal sialic acid.
Keywords: neutrophilic granulocytes, mononuclear leukocytes, phosphatidylinositol-3’-kinase, type 1 diabetes mellitus