Characterization of teic homycin A1 complex produced by Actinoplanes teichomyceticus NRRL-BL6726
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
Actinoplanes teichomyceticus is an important microorganism used for industrial production of teicoplanin, a “last line defense” glycopeptide antibiotic against multidrug resistant cocci. This strain also produces a second group of antibiotics collectively known as teichomycin A1 complex (TeiA). Here, the ability of A. teichomyceticus to accumulate TeiA in different media has been examined and structures of the major members of TeiA have been determined with the help of high-resolution mass spectrometry. TeiA is not produced in all tested media; moreover, different batches of nutrients (such as rapeseed and cottonseed flours) affected the ability of the strain to produce TeiA. Mass spectrometry experiments revealed that TeiA consists of 3 known and 1 novel moenomycins; the presence of other as-yet-unknown phosphoglycolipids cannot be ruled out due to their low production level and inherently complex biosynthetic pattern. It is likely that genes for early stage of moenomycin A biosynthesis are highly similar to TeiA biosynthetic genes, and their cloning can be pursued via homology-based searches.
Keywords: Actinoplanes, teichomycin A1, moenomycin, mass spectrometry