Analysis of variance of influence of thiosulphonic acid derivatives on the Nà+, K+-ATP-ase activity of loach embryos in vitro
H. Yaremkevych1, I. Polischuk2, S. Mandzynets2, M. Bura2, D. Sanagurskyi2, V. Lubenec1, V. Novikov1
1Lviv National Polytechnic University
12, S. Bandera St., Lviv 79013, Ukraine
2Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
The evaluation of influences of potassium salts of metyltiosulphonate and n-aminobenzentiosulphonate in the concentrations 10-3 ÷ 10-8 Ì (dose factor) on the Na+, K+-ÀÒP-ase activity in the early period of development (60–330 min.) of loach embryos (time factor) using of one- and two-factor analysis of variance has been performed. It has been detected, that the changes of enzyme activity are mainly caused by the action of the investigated biologically active substance and depended on embryos development time. The greatest sensitivity of Na+, K+-ATP-ase membranes of embryos to the changes in concentration of all investigated thiosulphonic acid derivatives existed during the development from 210 minutes to 270 minutes and the lowest one was at the stage of 16 blastomeres (150 min). The measure of the variability of activity of Na+, K+-ATP-ase membranes was determined and the influence of various concentrations of thiosulphonic acid derivatives, and the embryo development time factor. The conducted analysis of variance revealed a minor contribution of other unaccounted factors in the study of changes in the activity of the membrane enzyme – 2,7 ÷ 13,7% for potassium metyltiosulphonate and 3,8 ÷ 6,4% for potassium aminobenzentiosulphonate.
Keywords: loach embryo, Na+, K+-ÀÒP-ase activity, thiosulphonic acid derivatives, analysis of variance