Growth of actinomycetes in vivo and in vitro on the coal waste from central enrichment “Chervonohradska”

B. Ostash, Ň. Gren, S. Beshley, V. Baranov, V. Fedorenko*

Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine

Growth of actinomycetes on coal waste was investigated under conditions of the direct inoculation (in vivo) and submerged cultivation in the media containing the waste (in vitro). The size of population of Streptomyces sioyaensis remained virtually unchanged after 22 months of field experiment (end of June 2009 – May 2011) in the rejected coal within the rhizosphere of birch. In the same time Streptomyces sioyaensis titer dropped 3–4 orders of magnitude in wast samples without plant cover. In vitro, S. sioyaensis and four actinomycete isolates from birch Betula pendula rhizopshere demonstrated different efficacy of growth, which increased significantly under co-cultivation conditions.

Keywords: Streptomyces sioyaensis, actinomycetes, heavy metals, bioremediation

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