Role of the absA2gh gene in the regulation of moenomycin a production by Streptomyces ghanaensis ATCC 14672
M. Rabyk, H. Mutenko, B. Ostash, V. Fedorenko
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
Gene absA2gh encoding response regulator of two-component system AbsA2gh1/2 was identified on the chromosome of Streptomyces ghanaensis – producer of moenomycins. Overexpression of absA2gh in S. ghanaensis and heterologous expression in S. coelicolor led to decrease in MmA and actinorhodin production, respectively. In the same time, no significant changes in moenomycin production were revealed in S. ghanaensis ΔabsA2gh mutant. Thus absA2gh appears to influence moenomycin production under overexpression conditions, although it is not an essential regulator of this pathway.
Keywords: Streptomyces ghanaensis, moenomycin A, pleiotropic regulators, two-component system