Using the diagnostic methods to identify the infection degree of peach cultivars by Plum pox potyvirus

S. Martinov1, O. Mitrofanova1, I. Mitrofanova1,2

1Nikitsky Botanical Gardens National Scientific Centre NAAS of Ukraine
98648, Ukraine, Crimea, Yalta, Nikita
2Educational Scientific Center of Biology and Ecology of Subtropical Plants and Landscape Management of NULES of Ukraine
98648, Ukraine, Crimea, Yalta, Nikita
nikita@nauu.kiev.ua

The screening of infection degree of peach cultivars cased by Plum pox potyvirus (PPV) in the South and South-East of Ukraine has been carried out. Four areas infected by Plum pox potyvirus have been revealed and liquidated. The efficiency of complex using of diagnostic methods for evaluation of PPV infection degree of peach cultivars has been shown: indicator plants, Pirotest-ELISA system, immunochromatographic analysis (test strip), selection of tolerant and virus resistance cultivars with detection of steroid glycosides in organs and tissues of plants by the method of thin-layer chromatography.


Keywords: peach, plum pox potyvirus, PPV, Pirotest-ELISA system, immunoassay analysis, steroid glycosides


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