Oxygen transport function of hemoglobin under the admission agmatine in experimental diabetes mellitus
M. Lyuta, I. Ferents, V. Burda, A. Fedorovych, K. Dudok, N. Sybirna
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
The effect of agmatine – selective inhibitor of NO-synthase in the oxygen transport and spectral characteristics of rats hemoglobin with experimental diabetes induced by streptozotocin. In the case of intramuscular administration of agmatine diabetic animals with concentration of 20 mg / kg for 14 days, we observed changes in the affinity of hemoglobin for oxygen. Curve of oxyhemoglobin dissociation was shifted to the right with increasing the P50 and reduced alkaline resistant hemoglobin. Also was found that the administration of agmatine causes changes in the electron spectr of hemoglobin in the Sore band (350–450 nm). Agmatine as an endogenous regulator of the synthesis of NO can exert its influence on the affinity of hemoglobin for oxygen, spectral characteristics and oxygen transport of hemoglobin through the level of NO and its deposit with formation of S-nitroso and nitrosylhemoglobin.
Keywords: hemoglobin, oxyhemoglobin dissociation curve, the absorption spectr of oxyhemoglobin, agmatine, experimental diabetes