Viability of lower crustaceans under the influence of biologically active substances of biotechnological water from the medicinal leech (Hirudo verbŕna)
Ŕ. Frolov, R. Litvinenko
State Higher Educational Institution «Zaporizhzhya National University»
66, Zhukovskyi St., Zaporizhzhia 69600, Ukraine
The organism of the medicinal leech produces a complex of unique biologically active substances that are allocated into the aquatic environment with waste products and exhibit a wide number of therapeutic effects. The aim of the work was to study the influence of biologically active substances of biotechnological water from the leeches in artificial ecosystems on the survival of lower crustaceans (Daphnia magna Straus). We determined the acute lethal toxicity of biotechnological water from the medicinal leech on the test organism. Index of acute toxicity, compared with controls, in the first hours of the experiment (4–6 hours) is 3,3% and further increases, and starting from the third day (after 72 and 96 hours) acute toxicity was observed at the level 60,0 and 69,0%, respectively. Daphnids that were cultivated in biotechnological water from the medicinal leech differed morphologically from the control; in the initial stages of biotesting they had larger average body size and showed greater activity.
Keywords: biotesting, medicinal leech, biologically active substances, test-organism, acute toxicity