YtrA–like regulatory gene SSFG_05654 affects morphogenesis and antibiotic resistance of Streptomyces ghanaensis ATCC14672

O. Tsypik1Y. Dacyuk1, K. Flardh2, V. Fedorenko1, B. Ostash1

1Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
2Lund University
Lund 22362, Sweden

Gene SSFG_05654 encodes a transcriptional regulator of YtrA subfamily within the genome of moenomycin producer Streptomyces ghanaensis ATCC14672. The gene was replaced with apramycin resistance cassette. The resulting mutant S. ghanaensis 05654::Am was slightly delayed in aerial hyphae formation and spore pigment production when growing on oatmeal agar medium. On tryptic soy agar the mutant grew poorly and showed Bld-like phenotype. Antibiotic resistance assay revealed that S. ghanaensis 05654::Am is more sensitive to β-lactam antibiotics such as penicillin and ampicillin. SSFG_05654 disruption had no effect on the level of moenomycin production.

Keywords: Streptomyces ghanaensis, HTH transcriptional factors, morphogenesis, moenomycin

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