Comparison of activity of three heterologous promoters in Streptomyces ghanaensis ATCC14672

H. Mutenko1, M. Lopatniuk1, L. Horbal1, A. Luzhetskyy2, V. Fedorenko1, B. Ostash1

1Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
2Helmholtz Institute for Pharmaceutical Research Saarland, Saarland University Campus
Building C2.3, 66123 Saarbrucken, Germany

Streptomyces ghanaensis ATCC14672 produces moenomycin A, the only known natural product that directly inhibits peptidoglycan glycosyltransferases. It is desirable to develop reliable genetic tools for this strains which would allow to generate ATCC14672 derivatives producing novel moenomycins. Using β-glucuronidase reporter system, here we studied the activity in ATCC14672 of the three heterologous promoters extensively used in Streptomyces genetics gylP1/P2, aac(3)IVp and ermEp. The promoter of glycerol utilization operon gylP1/P2 displayed the highest transcriptional activity, while aac(3)IVp the weakest one. Activity of the latter was 2 and 3 times lower than that of gylP1/P2 on the first and fourth day of growth, respectively. The activity of ermEp was somewhat higher than that of aac(3)IVp, but did not exceed the strength of gylP1/P2.

Keywords: Streptomyces ghanaensis, moenomycin A, β-glucuronidase, reporter system

Full text (PDF)