With the rhizosphere celandine Chelidonium majus L. isolated 248 strains of actinomycetes. Investigated antibacterial and fungicidal properties of selected strains against bacteria and fungi of the genera Bacillus, Staphylococcus, Escherichia, Pseudomonas, Erwinia, Candida, Aspergillus. We found that 85% of strains of actinomycetes inhibited the development of an organism. Described strains that inhibit the growth of almost all used test cultures , except fungi of the genus Aspergillus, about 10% of isolates delayed growth of only one test culture. Some isolates, including strain Lv 28-52, Lv 28-110, Lv28- 142 , Lv 28-145, Lv 28-202, at a high level inhibited growth of yeast and pathogenic bacteria. Described in the actinomycetes strains may be potential producers of new bioactive substances.