Early increasing of ═22 content and peroxiredoxin and thioredoxin activities in the tissue culture of Arabidopsis thaliana under osmotic stress of different intensity

S. Jadko

╠.G. ╩holodny Institute of Botany, NAS of Ukraine
2, Tereschenkivska St., Kyiv 01601, Ukraine

Under the mild osmotic stress (10% PEG) in the tissue culture of A. thaliana the gradual increasing of the ═22 content and the peroxiredoxin (PR) and thioredoxin (TR) activities take place. These changes can not be relate to effects of an early stress oxidative burst and a ═22-induction signaling. However, under the acute osmotic stress (25% PEG), there is the early and high peak increasing of the ═22 content with subsequent the ═22-dependent increasing of the PR and TR activities and forming of the ═22-PR-TR stress signal system. At the same time the PR and TR, besides their antioxidant function, also participate in the acceptance and transduction of the ═22 redox signals.

Keywords: tissue culture, osmotic stress, ═22 signaling, peroxiredoxin, thioredoxin

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