PCR-analysis of promoter area Ppd-B1 gene in tetraploid wheat spacies T. Turgidum, T. Polonicum, T. Carthlicum
A. Muterko, I. Balashova, Yu. Sivolap
Plant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation
3, Ovidiopolska Road, Odessa 65036, Ukraine
The photoperiod insensitive of wheat is defined by allelic states of genes the Ppd-1 system. The DNA-marker for identification of haplogroup, haplotypes and mutations in promoter area Ppd-B1 gene has been proposed. By method of bioinformatic analysis on combination of mutants and intact alleles to respectively haplotypes have been defined. Inter-alleles associations and the phylogenetic structure of haplotypes has been studied. Is investigated of promoter area Ppd-B1 gene by PCR-analysis in 58 accessions of tetraploid wheat: T. turgidum, T. polonicum and T. carthlicum from 36 countries have been analysed. Haplogroup and spread frequency of haplotypes in species are investigated was defined.
Keywords: tertaploid wheat, Ppd-B1 gene, PCR analysis, haplotypes, phylogeny