ATPase activity of rat hepatocytes membrane under the influence of nicotinic acid adenine dinucleotide phosphate

M.Vergun, S.Bychkova

Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
s.bychkova@gmail.com

This paper studied the effect of nicotinic acid adenine dinucleotide phosphate (NAADP) on rat hepatocytes membranes ATPase active. We found a statistically significant reduction of specific Na+, K+-ATPase activity of microsomal fraction by (59,538,85)%, (p=0,05, n=7) at presence of NAADP. Also, it was found that NAADP reduces basal Mg2+-ATPase activity of rat hepatocytes membranes by (66,688,42)%, (p=0,001, n=9). However, it is shown that the specific Ca2+ -ATPase activity of microsomal fraction under the actions of NAADP increases by (71,656,43)% (p=0,017 n=8). It is speculated that NAADP-released calcium is actively transported by Ca2+-pumps as the endoplasmic reticulum and also by pumps of the plasma membrane and that require close localization of NAADP receptors and Ca2+-ATPase in membranes of rat hepatocytes.


Keywords: NAADP, ATPase activity, hepatocytes, microsomal fraction


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