Microbiota of the coal pit waste heaps of Chervonograd mining region

S. Kuzmishyna, S. Hnatush, O. Moroz, L. Karpinets, V. Baranov

Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
Institute of Ecology of the Carpathians, NAS of Ukraine
4, Kozelnytską St., Lviv 79026, Ukraine

Different groups of microorganisms from coal pit waste heaps of Chervonograd mining region are selected and the number of bacteria in each group is calculated. The dependence of the number of microorganisms from different groups both on the gangue (black or red), and on the place of sampling (terrace, top or base), existence of vegetation, and soil humidity is shown. The dominance of oligonitrophilic bacteria and microscopic fungi was established. The largest number of microorganisms was found on the waste heaps under the mosses. We observed the high quantity of cellulose decomposing aerobic bacteria in all gangue samples under investigation. Presence of thiobacteria in the samples indicates the processes of sulfur minerals oxidation with formation of sulfate acid. Certain amount of sulfate and sulfur reducing bacteria indicates the activity of sulfur cycling processes. The difference between the composition and the number of microorganisms from different groups selected from red and black gangue samples is shown. The analysis of the red gangue microbiocenosis showed the significant dominance of microscopic fungi, including yeasts and oligonitrophilic bacteria. The black gangue samples contain representatives from all groups of microorganisms under investigaton.

Keywords: microbiocenosis (microbiota), coal pit waste heaps, microscopic fungi, cellulose decomposing aerobic bacteria, oligonitrophilic bacteria, sulfate and sulfur reducing bacteria, colorless sulfur oxidizing bacteria

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