Verapamil influence on secretory function of stomach in case of long-term omeprazole-induced hypochlorhydria
O. Tsyryuk, Y. Golovinska, N. Skochko, T. Beregova
Educational and Scientific Centre “Institute of Biology” Taras Shevchenko National University of Kyiv
2, Academician Glushkov Ave., Kyiv 03022, Ukraine
In this work gastric secretion in rats was investigated after long-term separate injection of H+-K+-ATPase blocker(omeprazole) and its simultaneous administration with calcium receptor blocker (verapamil). Also gastrin level in plasma was measured. The study was carried out on white nonlinear rats. Rats were divided into 4 groups. The 1st group was the control, rats were injected by water (0.2 ml). Omeprazole (14 mg/kg) was administered intraperitoneal to the rats of 2nd group. Verapamil was administrated orally to the rats of 3rd group. Animals of 4th group was simultaneous administrated with omeprazole and verapamil. All drugs were administered for 28 days long. In 24 hours period after last injection gastric basal acid output was measured in acute experiments by method of perfusion of isolated stomach by Ghosh and Shild. At the end of experiments blood samples were collected to determine gastrin consentration in blood plasma. It was established that simultaneous administration of omeprazole and verapamil suppressed the stimulated gastrin secretion by G-cells of the stomach, due to the increase of pH after H+-K+-ATPase blocker usage. Besides calcium receptors blockade prevented hyperplasia of ECL cells, inhibited gastric secretion and prevented hypersecretion that was observed one day after prolonged injection of acid reducing drugs.
Keywords: gastric secretion, omeprazole, verapamil, gastrin