Dynamics of free-radical processes and the level of endogenous intoxication in rats under conditions of short-term occlusion of the pancreatic duct

V. Makarchuk

1SI «Institute of Gastroenterology of National Academy of Medical Sciences of Ukraine»
96, Gazety “Pravda” Ave., Dnipropetrovsk 49074, Ukraine

Experiment on laboratory rats showed that as a result of surgical occlusion of pancreatic duct by ligation with “Catgut” 4/0 on the 6th day of study acute pancreatitis has been developed. After resorption of ligatures (10 days) passability of the pancreatic duct was restoring. On day 30th of the experiment the renewal of acinar tissue has been observed with only minor fibrotic changes in the organ. On the basis of morphological study all changes has been classified into three stages: inflammatory-necrotic stage of acute pancreatitis after 6 days, regenerative-proliferative stage after 15 days, and recovery stage after 30 days of experiment. Determination of biochemical markers of fibrosis in the rat blood confirmed the results of morphological studies. By the end of the second week of the experiment the maximum intensification of lipid peroxidation took place, which contributed to the formation of secondary products of lipid peroxidation in the blood. An increase in the level of endogenous intoxication has been shown, expressed byaccumulation of medium-weight molecules in rat plasma, growth of nuclear, toxic fractions and fractions of ñhromîphore.

Keywords: TBA-active products, molecules with average weight, protein-bound hydroxyproline, free hydroxyproline, pancreas

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