The activity of enzymes of glutathione antioxidant system of Desulfuromonas acetoxidans IMV B-7384 under the influence of ferric (III) citrate
O. Maslovska, S. Hnatush, S. Katernyak
Ivan Franko National University of Lviv
4, Hrushevskyi St., Lviv 79005, Ukraine
Desulfuromonas acetoxidans ≤ŐV ¬-7384 bacteria are highly perspective microorganism for construction and development of microbial fuel cell technologies aimed for wastewater remediation from transition metal ions. However, metal ions catalyze formation of reactive oxygen species that could harmfully affect bacterial cells. Activity of glutathione peroxidase, glutathione-S-transferase and glutathione reductase of D.†acetoxidans IMV B-7384 bacteria was investigated under the influence of different concentrations of ferric (III) citrate. Addition of metal salt into cultural medium caused the increase of activity of all investigated enzymes in comparison with control. The activity of glutathione peroxidase, glutathione-S-transferase and glutathione reductase changed dependently on metal salt concentration and duration of bacteria cultivation. The activity of glutathione peroxidase and glutathione-S-transferase decreased with increasing of duration of bacterial cultivation while activity of glutathione reductase increased under the influence of investigated metal salt. Decreasing of activity of glutathione peroxidase and glutathione-S-transferase could be caused by decreasing of reduced glutathione content in bacterial cells under the influence of ferric (III) citrate. Probably, quantity of reduced glutathione produced by glutathione reductase was not sufficient to maintain the necessary level of this tripeptide in bacterial cells under the influence of ferric (III) citrate. Enhancement of activity of enzymes of glutathione system confirms their necessary role in antioxidant protection of D. acetoxidans IMV B-7384 cells.
Keywords: Desulfuromonas acetoxidans, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, ferric (III) citrate