Proliferative activity of maize roots meristems under the action of herbicides and hyperthermia
N. Pavlyukova, L. Boguslavskaya
Oles Honchar National University of Dnipropetrovsk
72, Gagarin Ave., Dnipropetrovsk 49010, Ukraine
It was investigated the separate and compatible action of herbicide dimethenamid and the elevated temperature on the proliferative activity of meristematic cells of maize roots. It was determined that the studied factors inhibit root growth due to decreased primarily mitotic activity of meristem cells. It was established the negative influence of herbicide separately and in conjunction with elevated temperature on growth of root maize throughout the period of germination, which in this case was determined after 24 hours by hyperthermia, and after 48 hours – by herbicide. At the same time the frequency of various violations (output of aberrant cells, pyknotic nuclei) in mitotic cells during the whole germination period was determined by dimetenamid. It has been defined a level of chromosomal instability which is largely determined by the influence of dimethenamid. Analyzing the changes in aberrations index and the percentage of cells with pyknotic nuclei for the group of aneugen violations that were observed in mitotic cells is found that the combined effect of herbicide and high temperature increases the number of aberrant cells, and temperature – the number of cells with nuclei pyknotic.
Keywords: maize, meristem, dimethenamid, hyperthermia, mitotic activity, aberrations, pyknosis