Researching of complexes antisense oligonucleotides with the newly-synthesized carriers based on DMEM

N. Mikush1, V. Vlizlo1, O. Zaichenko2, R. Stoyka3, D. Ostapiv1, I. Petruh1

1 Institute of Animal Biology, NAAS of Ukraine
38, V. Stus St., Lviv 79034, Ukraine
2 Universitet Lviv Polytechnic
¾, Sheptitskyi St., Lviv 79010, Ukraine
3 Institute of Cell Biology
16, Dragomanov St., Lviv 79010, Ukraine

Prion infections are caused by specific pathological form of the prion protein, which is present in all mammals. It is known that organisms that do not contain the cellular prion are resistant to the development of prion infections. Prion removal from organism can be achieved by blocking the translation of its mRNA without intervention into the genome. An obstacle to the use of oligonucleotides for the reduction of PrPC in brain is their impermeability through the blood-brain barrier, so the actual problem is to provide effective methods and means upravlyaeniya transport oligonucleotides into cells of the CNS. The article contains results of researching, the newly-synthesized polymeric carriers based on dimethylaminoethyl methacrylate in conjunction with antisense oligonucleotides (asODN). According to the research have been selected effective sequence asODN and polymeric carriers for inhibiting expression of the physiological prion in lab animals. Was established, that using of new polymers carriers reduces the amount of prion in organism for a certain time, which can be used in the treatment and prevention of prion infections. Also have been described the results studies of cytotoxic properties of the newly-synthesized cationic-active polyelectrolytes and their impact on the systems of the organism laboratory rats.

Keywords: prion, oligonucleotide, brain barrier, dimethylaminoethyl methacrylate, gene expression

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