Effects of chlorpyrifos intoxication on biochemical and erythrocytic parameters of rats blood
Y. Salyha, V. Rosalovsky
Institute of Animal Biology, NAAS of Ukraine
38, V. Stus St., Lviv 79034, Ukraine
Chlorpyrifos is an organophosphate compound. It is dangerously toxic, but the underlying mechanisms of its toxicity are not fully understood yet. We studied the effects of 30 mg/kg chlorpyrifos exposure to rats on certain biochemical parameters of their blood, measured at 1, 3, 6, and 10 days after the exposure. Changes in aspartate and alanine aminotransferases, alkaline phosphatase and protein metabolism indices, along with a decrease in butyrylcholinesterase activity in the blood serum were found, showing how multidirectional are the toxic effects of chlorpyrifos, which mechanisms are not limited to the well-known anticholinergic action. Studying hematological parameters of intoxicated rats, we found a decrease in hemoglobin concentration and acid hemolysis resistance of red blood cells in the blood of intoxicated rats, compared with intact animals, during the experimental period. Identified changes of enzymatic activities, indicators of protein metabolism in blood and red blood cell parameters of studied animals under the chlorpyrifos action can be used as additional indicators in evaluation of toxic effects of this and other organophosphorus compounds in mammals.
Keywords: chlorpyrifos, toxicity, red blood cells, blood, rats