Pseudomonas aeruginosa PA01 biofilm formation dynamic in presence of the meso-tetra(4-n-methylpiridyl)porphyrine bismuth complex
Odesa National Mechnikov University
2, Dvoryanska St., Odesa 65082, Ukraine
Biofilms growth dynamic discovery at the first hours of incubation were carried out on 24×24 ěě cover glasses that were placed in 35 ěě Petri dishes containing 2 ml of the Gis media with P. aeruginosa cells (103 CFU/ml) and 0,4 or 40 µM of the meso-tetra-(4-N-methyl-piridyl)porphiyrine bismuth complex (Bi3+-TPP). Petri dishes with cover glasses were incubated from 15 min to 4 hours at 37°Ń. After incubation all cover glasses were washed with physiological solution, fixed with 96% ethanol for 10 minutes, and stained with crystal violet for 5 minutes. Cover glasses were examined with a microscope Primo Star PC, Carl Zeiss on × 900 and photographed with Olympus DCM (3,0 M pixels) camera. For biofilm growth dynamic discovery at time interval from 1 to 7 days biofilms were obtained in 48-well «Nunclon» polystirol plates after preincubation with 0,4, 40 and 80 µM of the Bi3+-TPP. Obtained results show that discovered substances in all concentrations violates the biofilm growth dynamic. It was shown that Bi3+-TPP presence in culture medium delays a microcolony formation at the first hours of incubation. Discovered substance also demonstrated the ability to delay the development of P. aeruginosa PA01 biofilm on the time interval from 1 to 7 hours. In this condition in presence of each concentrations of the discovered substance biofilm development stops at third day.
Keywords: Pseudomonas aeruginosa PA01, meso-tetra(4-N-methyl-piridyl)porphyrine bismuth complex, biofilm, biofilm growth dynamic