The siderophores formation by Pseudomonas aeruginosa cells under the “Bacteriphage pseudomonas aeruginosa” preparation influence
I. I. Mechnikov National University of Odessa
2, Dvorianska St., Odessa 65058, Ukraine
There was studied that the most intensive siderophore production which is one of pathogenicity factors was typical for Đseudomonas aeruginosa ATCC 15692 and ŔŇŃŃ 9027 strains. The addition of 3 µM iron (III) ions practically didn’t change the corresponding characteristics, but 30 µM have caused 1,5- 2,5-fold suppression of chelator production, 300 µM – completely stopped their synthesis. The «Bacteriophage Pseudomonas aeruginosa» preparation, that had lytic activity against P. ŕeruginosa ATCC 15692 and ŔŇŃŃ 9027 strains, and pseudolytic one against ATCC 27853 and ATCC 10145 cultures, significantly influenced on siderophore production. The chelator synthesis was suppressed as compared to the baseline values while simultaneous introduction of microorganisms and the preparation in the culture medium was carried out. But, in this case, when the concentration of iron (III) ions was increasing the siderophore production, conversely, was becoming more intensive, especially for P. aeruginosa ATCC 27853 and ATCC 10145 strains. The preparation addition at 48 hrs after microbial cultivation initiation caused siderophore synthesis intensity decreasing with Fe3+ concentration raising, but the obtained values were higher than when the microorganisms are not proved to the bacteriophage “pressure”.
Keywords: iron (III) ions, siderophores, “Bacteriophage Pseudomonas aeruginosa” preparation, Pseudomonas aeruginosa